Ps. I have no comment for Mike R as well. He would whoop my ass in a typing war and I know better not to mess.

yeah i sense Mike would kick my ass too.

Regarding my comments we all have to wait and see stitch i’m only watching the actions of individuals who were relevant in the apprehension of GSK.

Consider it gossip, i know something i shouldn’t know

yeah i sense Mike would kick my ass too.

Regarding my comments we all have to wait and see stitch i’m only watching the actions of individuals who were relevant in the apprehension of GSK.

Consider it gossip, i know something i shouldn’t know

Okay think I got what you mean now Simplicity and you have inside information. Lol about Mike R.

Hi,

Well, IF some scientifically viable, matching, verifiable DNA has been developed, I seriously doubt it was developed from saliva, because it seems to me that they would have developed that years ago. If there is some low cell count DNA from MULTIPLE letters, then maybe they are onto something. The info here is so cryptic that it is hard to tell anything. But given what happened to me in 2002, I’ll wait and see how this all plays out.

Mike

Actually, they have developed some sophisticated post-PCR purification techniques that are really sensitive. Woth standard PCR, you need about 20-50 cells’ worth of DNA to raise a profile. I know they have Montage filters now. They can do longer run times; they can increase the temperature; they can increase the number of cycles they do the PCR. These are some of the examples of newer techniques that many labs have validated for use. As a defense lawyer, I can poke holes in all of these techniques, but they are certainly useful for initial identification. Their sensitivity can be as low as one cell’s worth of DNA
There is no lower amount than one cell. So, we’ve reached the level of maximum efficiency with these techniques. I’ve seen post-PCR purification techniques take a sample that did not raise a conventional profile to one that valid allele calls on each locus. It is amazing what they can do now. And, by the way, the technology I’m talking about was state-of-the-art about 5 years ago. There is no telling what new developments have arisen.

So, if they still have their samples from the earlier testing, they only need to get one cell off of the sample to be able to raise a valid profile for Zodiac. The testing is really not the limiting factor. Storage conditions are. Whether untested samples remain is. The techniques are unquestioned. It’s the circumstances surrounding the testing that are potentially problematic.

The point isn’t pcr purification techniques. It is this, and the point is very simple: Even after all the facts I and others have discovered since 2007, people still blithely say that Z licked his stamps and envelopes. I think even Poyser said this. Had the letter writer done so, they would have recovered his DNA long before now using the same primitive techniques they used in 1998 or so to easily develop DNA from the two letters keel described as being forgeries.

Now they are using low cell count techniques. If they get just one cell from one letter, how do we know it belonged to the letter writer? I’ve wised up a lot since 2002 when I got clobbered by that phony DNA before a national TV audience. If VPD or anyone else is going to say they have the letter writer’s DNA, they had better be ready to say how many letters they found it on. If it is just one, then that is a problem because we all know that the more sensitive the technique the more likely it is to magnify a single cell of contaminant dna. So these new techniques are double edged swords when used on old, contaminated evidence.

Mike

a huge downside of this would be the great upside that came with this development and that is the naming of Jane and John doe for the family’s . closure to some degree for surviving family members

Hi,

I think it should be clear to any scientist that given the level at which they are now allegedly looking for cells (a single cell on a letter that has been handled by many, many people, that they must recover DNA from multiple letters (even if they do isolate the glue side in their sampling) just to be abundantly cautious and verify that they have the letter writer’s DNA. For the umpteenth time, had Z licked these letters and stamps, we would not be having this conversation because there would have bene so many cells on the letters that DNA would have been recovered years ago.

Mike

But having DNA is only useful if you have someone to compare it to (either directly or through relatives). The question is, of course hypothetically speaking, if they had DNA and have not been able to trace it to anyone would they still announce that they had it ?

Well lets just PRAY that they got what they needed with Z’s DNA out of GED Match before this happened.

Hi Tommy,

So is it now a given that someone has isolated genuine 100% verified Zodiac DNA. Is that correct? We know that this is not another 2002 political hatchet job? Can u say how u know this? Is the source within LE or otherwise in a position to know?

Mike

Hi Tommy,

So is it now a given that someone has isolated genuine 100% verified Zodiac DNA. Is that correct? We know that this is not another 2002 political hatchet job? Can u say how u know this? Is the source within LE or otherwise in a position to know?

Mike

I know this isn’t directed at me but i think you’ve highlighted rather well all the reason why a Z profile will probably never be likely.

Whatever lead i am following is pretty darn close to finding their man, i know a female was sought approx a month ago whether or not she supplied her DNA is another thing.

My guess with all things considered is probably the Santa Rosa Hitcher murders with a slim chance they discovered a overlap between Z and SRH if that were even possible?

Anyway, if i tip off the culprit and he blows his brains out cool hope he misses

One more thing, nothing i have done is illegal, no hacking, no stalking nothing.

Gedmatch works in a certain way, the database of family trees etc is normally freely available to all.

There’s a bit of guess work here and there but eventually if you know what your doing you will stumble apon things and from there you get the picture.

We see what happens fingers crossed, It be interesting to know the search history of names on these forums in recent times or a SRHM site.

Mike, regarding stamp licking etc would you think that this department could play any role or in the process of obtaining such cells? even by means of a innovative approach? https://depts.washington.edu/gemsec/about/index.html

You are more knowledgeable than i about the specifics.

Hi Simplicity,

This is confusing:
"Whatever lead i am following is pretty darn close to finding their man, i know a female was sought approx a month ago whether or not she supplied her DNA is another thing."

You’ve indicated that you know something that therest of us don’t. I’ve seen your posts and am wondering what they mean lol. I thought you were tied into LE and knew what VPD is up to. But now I’m not so sure. Can u tell me if your research is leading in the direction of the a car or the Santa Rosa case?

As far as your question goes, my position is that Zodiac did not lick the letters. Therefore the best spit scientists in the world can’t help lol.

Mike

You’ve indicated that you know something that therest of us don’t. I’ve seen your posts and am wondering what they mean lol. I thought you were tied into LE and knew what VPD is up to. But now I’m not so sure. Can u tell me if your research is leading in the direction of the a car or the Santa Rosa case?

Not tied with LE nor any genealogists relevant the latter isn’t interested with my good intentions, i suspect haunting them on ged might get me tied by LE.

Regarding the direction, we start at the top, originates from california and of “high value” due to extensive work being carried out. The highlighting of Vietnam suggests the era, the pinpointing of a female suggests how close.

The speculation part, there might be a overlap between this female and the Z case, she wasn’t a victim.

Now whatever it is that i’m observing would be insanely difficult to do on the inside, To do it from a computer from the other side of the world with out any kind of hacking or illegal activity and talk with out annoying anyone here with my conjectures is another, i tread softly.

Also, theres literally no point in providing the names relevant they are only on the peripheral, My searches here has crossed paths with Soze works which again alludes to the era, place and theme and nothing more.